Experimental Setup for Irradiation of Cell Cultures at L2A2

Laser–plasma proton sources and their applications to preclinical research has become a very active field of in recent years. In addition small dimensions as compared classical ion accelerators, they offer the possibility study biological effects ultra-short particle bunches correspondingly high dose rates. We report on design an experimental setup for irradiation cell cultures at L2A2 laboratory University Santiago de Compostela, making use 1.2 J Ti: Sapphire laser with 10 Hz repetition rate. Our comprises energy separator consisting two antiparallel magnetic fields realized by set permanent magnets. It allows selecting narrow window around adaptable value 5 MeV out initially broad spectrum typical Target Normal Sheath Acceleration (TNSA). At same time, unwanted electrons X-rays are segregated from protons. This part is located inside target vessel laser. A subsequent vacuum flange sealed thin kapton passage external sample irradiation. combination passive detector materials real-time monitors applied measurement deposited radiation dose. critical point this interdisciplinary project manipulation samples under well-controlled, sterile conditions. Cell prepared flasks ultra-thin entrance analysed nearby Fundación Pública Galega Medicina Xenómica IDIS. The first trials will be centred quantification DNA double-strand breaks function